Purification of the hexameric Smc5/6 complex from S. cerevisiae

Cole-Parmer CG-450 Freezer/MillSpex Sample Prep laboratory mills: 6770 Freezer/MillSpex Sample Prep laboratory mills: 6870 Freezer/MillSpex Sample Prep laboratory mills: 6870D Freezer/Mill

When purifying yeast, the tough cell wall makes efficient cell lysis difficult. As an alternative to cost-intensive enzymatic removal, mechanical lysis by freeze-grinding can be used. This application note demonstrates this method using the example of S. cerevisiae.

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