Cole-Parmer CG-900 Cryo-Blade Cryogenic Knife Mill

Product Description

Due to legal requirements, it is generally necessary in food analysis to grind a large amount of starting material, if possible without the loss of volatile substances, in order to obtain a representative sample for analysis. The CG-900 Cryo-Blade cryogenic knife mill from Cole-Parmer overcomes precisely this bottleneck in sample preparation.

Plant (fruits, vegetables, leaves) or soft animal sample material (organs, muscles) with a maximum quantity of 1 kg are ground under cryogenic conditions (-176°C using liquid nitrogen) within a short time to a fine powder for further analysis, e.g. with the QuEChERS method. The time-consuming grinding of small quantities and subsequent combining to obtain a representative population is therefore no longer necessary – the analysis can begin without any further intermediate steps. The sample only needs to be pre-ground into pieces no larger than 5cm. During the automatic filling of the double-walled grinding container with liquid nitrogen (patent pending), neither the laboratory staff nor the sample has direct contact with the cooling medium. Alternatively, the grinding container can also be filled manually with dry ice. Once the sample has been ground, both the grinding container and the lid of the container can be cleaned in the laboratory dishwasher without removing the blade.

The Cryo-Blade is programmed using a user-friendly touchscreen, with 9 different languages available. All grinding parameters can be freely programmed and 10 programmes can be saved.

The Cole-Parmer CG-900 Cryo-Blade enables fast and efficient sample homogenisation in the areas of pesticide analysis of foodstuffs – QuEChERS, DNA/RNA and protein extraction, analysis of genetically modified organisms (GMOs) and tissue disruption.

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Specifications

Grinding time: typically 15 minutes or less

Sample cooling: liquid nitrogen (automatic, Dewar flask required) or dry ice

Operation: touchscreen

Max. grinding capacity: 1 kg

Pre-cooling time: approx. 10 minutes (depending on the sample)

Blade speed: 120 – 3600 rpm

Mains connection: 230 V / 50 Hz

Dimensions: 70 cm height x 45 cm width x 63 cm depth

Weight: 50 kg (without grinding jar)

Options and Accessories

• • Sample clamps with quick-release fasteners for single tubes and for up to 6 deep-well plates

• Optimised DNA isolation kits for up to 75 reactions per kit. Available for DNA isolation from animal and plant samples.

• Deep-well plates with 96 or 48 wells, round or square wells

• Cover mats and films for deep-well plates

• Individual tubes in various designs and materials, with volumes of 0.6, 1 and 2 ml, 5 ml, 15 ml or 50 ml, with suitable sample racks, pre-loaded with grinding balls or beads, or empty

• Various grinding media (stainless steel, ceramic, silica, zirconia, etc.) in different diameters and shapes (beads, balls or rods)

• Cryo adapters for deep-well plates and individual tubes for cell lysis under cooled conditions, as well as a cryo station for pre-cooling the fully loaded adapters in liquid nitrogen

• Filling station for 4 mm stainless steel grinding beads for 96-well deep-well plates

Applications