Cole-Parmer HG-400 MiniG® Cell Disruption Mill

Product Description

The special mill Cole-Parmer HG-400 MiniG® for high-throughput cell disruption and fast and efficient sample homogenisation overcomes the bottleneck in sample homogenisation and extraction by combining both steps.

In particular, the variable use of either proprietary or customised deep well plates (up to 2 x 96 samples) or single tubes with 1 to 2 ml (up to 2 x 24 samples), 15 ml (up to 12 samples) or 50 ml (up to 6 samples) in suitable racks makes the Cole-Parmer HG-400 MiniG® cell disruption grinder, as well as the larger device, the HG-600 Geno/Grinder® 2010, unique among cell disruption devices. Disposable vessels are available to prevent contamination and save time. In addition, the user can choose from numerous different separate grinding media (beads, balls or pins) as well as digestion kits with pre-prepared vessels filled with sample-specific grinding balls or beads for homogenising a variety of sample types. Depending on the sample type, the user can select kits with ceramic beads, steel beads, glass beads or bead mixtures. These optimised kit combinations for efficient cell disruption and subsequent nucleic acid isolation guarantee the highest yields of high-purity RNA, DNA or proteins and other biomolecules.

The deep-well plates or individual tubes, filled with sample material and grinding media and suitably sealed, are placed in the racks provided in just a few simple steps and stacked and locked in the height-adjustable holder of the Cole-Parmer HG-400 MiniG® cell disruption mill as required. The user simply selects the digestion time in seconds or minutes and the speed and presses the start button. The exclusively vertical movement of the sample tubes supports a homogeneous and reproducible digestion result across all individual tubes. In addition, the extremely fast cell disruption due to the very high speed (500 – 1,500 oscillations per minute) protects the components to be extracted and ensures complete grinding even of difficult materials.

The Cole-Parmer HG-400 MiniG® cell disintegration mill offers monitoring of the disintegration by visual inspection throughout the entire process. The pulping process ends automatically after the time has elapsed, but can be interrupted manually by the operator at any time. A safety mechanism immediately interrupts the digestion process if an attempt is made to open the lid of the device.

If the samples also need to be kept cold during homogenisation, the user can choose from a number of different cryo-adapters for all available sample vessels. These are loaded with the already filled sample vessels and pre-cooled in liquid nitrogen for a few minutes in a cryo-station provided for this purpose. The aluminium cryo-adapters keep the sample cold during the grinding process, ensuring continuous cooling and protection of temperature-sensitive samples during the homogenisation procedure. Typical samples include human and animal tissues, plants and plant parts, cell cultures, fruits, vegetables and grains, cannabis, seeds, yeasts and bacteria.

A key application area for the Cole-Parmer HG-400 MiniG® cell disruption mill is sample homogenisation and extraction for pesticide analysis using the QuEChERS method. The QuEChERS method is mainly used for the analysis of fruit and vegetables – the method includes a number of mixing and extraction steps as well as preparatory sample homogenisation. These time-consuming and labour-intensive steps can be combined with the Cole-Parmer HG-400 MiniG® and processed with a higher throughput. When using the HG-400 MiniG® cell disruption grinder, the yield of pesticide is significantly higher and therefore the extraction is much more effective than when working manually. In addition, operator-dependent influencing factors such as shaking technique, shaking time etc. are eliminated and standardised. Sample throughput is increased and reproducibility is improved.

The Cole-Parmer HG-400 MiniG® cell disruption mill enables fast and efficient sample homogenisation in the areas of pesticide analysis of foodstuffs – QuEChERS, DNA/RNA and protein extraction, analysis of genetically modified organisms (GMOs) and tissue disruption

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Specifications

Options and Accessories

• Deep-well plates with 96 or 48 wells, round or square wells

• Cover sheets and films for deep-well plates

• Individual tubes in various designs and made from different materials, with capacities of 0.6, 1 and 2 ml, 5 ml, 15 ml or 50 ml, with suitable sample racks, either pre-loaded with grinding balls or beads or empty

• Various grinding media (stainless steel, ceramic, silica, zirconia, etc.) in different diameters and shapes (beads, balls or rods)

• Cryo adapters for deep-well plates and individual tubes for cell lysis under cooled conditions, as well as a cryo station for pre-cooling the fully loaded adapters in liquid nitrogen

• Filling station for 4 mm stainless steel grinding beads for 96-well deep-well plates

Applications